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In vitro & in vivo library screening

CRISPR/Cas9 Based Library In Vitro Screening

  • Customize CRISPR library and experimental design
  • sgRNA synthesis, cloning and library QC
  • Virus production and cell line infection
  • Perform specific cell selection (positive or negative selection)
  • Target identification through next generation sequencing (NGS) and data analysis, comparing sgRNA amplitude before and after selection
  • Target validation in cells in combination with other tools such as lentiviral gene overexpression, RNAi knockdown

 

 

In Vivo Target Identification and Validation

  • Customize CRISPR library and expand infected cells (cancer cells or T cells)
  • After in vivo screening, isolate tumor cells or T cells for NGS

 

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